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Please use this identifier to cite or link to this item: http://dspace.utalca.cl/handle/1950/10210

Title: Tracking Endogenous Amelogenin and Ameloblastin In Vivo
Authors: Jacques, J.
Hotton, D.
De la Dure-Molla, M.
Petit, S.
Asselin, A.
Kulkarni, AB.
Gibson, CW.
Brookes, SJ.
Berdal, A.
Isaac, J.
Keywords: ALVEOLAR BONE
STEM-CELLS
PERIODONTAL-LIGAMENT CELLS
ENAMEL MATRIX PROTEINS
PRIMARY RNA TRANSCRIPT
RAT INCISOR
SUPRAMOLECULAR STRUCTURES
RECOMBINANT AMELOGENIN
PROGENITOR CELLS
BONE-FORMATION
Issue Date: 16-Jun-2014
Publisher: PUBLIC LIBRARY SCIENCE
Citation: PLOS ONE 9 (6)
Abstract: Research on enamel matrix proteins (EMPs) is centered on understanding their role in enamel biomineralization and their bioactivity for tissue engineering. While therapeutic application of EMPs has been widely documented, their expression and biological function in non-enamel tissues is unclear. Our first aim was to screen for amelogenin (AMELX) and ameloblastin (AMBN) gene expression in mandibular bones and soft tissues isolated from adult mice (15 weeks old). Using RT-PCR, we showed mRNA expression of AMELX and AMBN in mandibular alveolar and basal bones and, at low levels, in several soft tissues; eyes and ovaries were RNA-positive for AMELX and eyes, tongues and testicles for AMBN. Moreover, in mandibular tissues AMELX and AMBN mRNA levels varied according to two parameters: 1) ontogenic stage (decreasing with age), and 2) tissue-type (e. g. higher level in dental epithelial cells and alveolar bone when compared to basal bone and dental mesenchymal cells in 1 week old mice). In situ hybridization and immunohistodetection were performed in mandibular tissues using AMELX KO mice as controls. We identified AMELX-producing (RNA-positive) cells lining the adjacent alveolar bone and AMBN and AMELX proteins in the microenvironment surrounding EMPs-producing cells. Western blotting of proteins extracted by non-dissociative means revealed that AMELX and AMBN are not exclusive to mineralized matrix; they are present to some degree in a solubilized state in mandibular bone and presumably have some capacity to diffuse. Our data support the notion that AMELX and AMBN may function as growth factor-like molecules solubilized in the aqueous microenvironment. In jaws, they might play some role in bone physiology through autocrine/paracrine pathways, particularly during development and stress-induced remodeling.
Description: Univ Talca, Dept Stomatol, Unit Periodontol, Talca, Chile; Jacques, J (Jacques, Jaime)
URI: http://dspace.utalca.cl/handle/1950/10210
ISSN: 1932-6203
Appears in Collections:Artículos en publicaciones ISI - Universidad de Talca

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