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Please use this identifier to cite or link to this item: http://dspace.utalca.cl/handle/1950/1672

Title: Aspects of isolation underpinning mitotic behaviour in lupin protoplasts
Authors: Sinha, A.
Caligari, P.D.S.
Keywords: cell- wall regeneration
Glycine - Max L
plant - regeneration
mesophyll protoplasts
Brassica - napus
somatic embryogenesis
culture media
Issue Date: 2004
Publisher: Csiro Publishing
Citation: Australian Journal of Botany 52 (5): 669-676
Abstract: This study reports on the influence of critical isolation factors on the subsequent culture of protoplasts of Lupinus albus L. Protoplasts were isolated from in vitro seedling cotyledons of five early maturing accessions in which protoplast yields and division frequencies appeared to be correlated as a high protoplast yield corresponded with a high division frequency. The overall difference among the accessions for mitosis was non- significant, although the highest yield and division frequency were observed in accession LA132, with Alban giving a significantly lower level. Accession Lucrop produced the lowest number of protoplasts, all of which collapsed during culture. Of the enzyme types used for tissue maceration, Pectolyase Y23, was significantly inferior to Macerase in terms of giving way to mitosis. The extent of division in Macerase- isolated protoplast population was 266% higher than that in the Pectolyase Y23- isolated one. The physiological maturity level of the explant, expressed in terms of developmental age, was optimal when 14 - 18- day- old seedling cotyledons were used for protoplast production and culture, rather than more mature ones, despite higher protoplast yields in the latter. On K8p medium, the protoplast division frequency was 129% greater when 18- day- old seedling cotyledons were used, than that with any other treatment. This work on protoplast culture of the potentially important lupin species, which is a pulse rich in dietary protein, oil and fibre, allows a further understanding of the biology, with an aim to advance lupin biotechnology.
Description: Caligari PDS (reprint author). Instituto de Biología Vegetal y Biotecnología, Universidad de Talca, 2 Norte 685, Talca, Chile.
URI: http://dspace.utalca.cl/handle/1950/1672
ISSN: 0067-1924
Appears in Collections:Artículos en publicaciones ISI - Universidad de Talca

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