DSpace About DSpace Software
 

DSpace Biblioteca Universidad de Talca (v1.5.2) >
Dirección de Programas de Investigación y Asistencia Técnica (DIAT) >
Artículos en publicaciones ISI - Universidad de Talca >

Please use this identifier to cite or link to this item: http://dspace.utalca.cl/handle/1950/4069

Title: In vitro antibacterial activity of the peptide PsVP-10 against Streptococcus mutans and Streptococcus sobrinus with and without glycocalyx
Authors: Padilla, C.
Lobos, O.
Hubert, E.
Poblete, F.
Navarro, A.
Nunez, L.
Keywords: Bacteriocin PsVP-10; Streptococcus sobrinus; Streptococcus mutans; Glycocalyx; Susceptibility
Issue Date: 2006
Publisher: Elsevier B.V. and the International Society of Chemotherapy
Citation: International Journal of Antimicrobial Agents 27(3): 212-216
Abstract: Abstract The antibacterial activity of the peptide PsVP-10 obtained from Pseudomonas sp. R10 against Streptococcus mutans and Streptococcus sobrinus was investigated. One hundred and twenty strains of S. mutans and 120 strains of S. sobrinus with and without glycocalyx were isolated from saliva samples in trypticase–yeast–cysteine–sucrose–bacitracin (TYCSB) agar. Bacterial identification was made by polymerase chain reaction. Glycocalyx production was observed in modified TYCSB agar and confirmed with a modified version of the microplate adherence assay. The minimum inhibitory concentration (MIC) of PsVP-10 bacteriocin was determined by means of the agar dilution method, and the time of bacterial death was calculated by means of colony-forming unit counts. The MIC of the bacteriocin PsVP-10 for both bacterial species with and without glycocalyx was <2 mg/L and the time of bacterial death was less than 240 s for all the studied bacterial strains. Thus, bacteriocin PsVP-10 could be an interesting possibility to combat these cariogenic bacterial species.
Description: Padilla, C.; Lobos, O. Laboratorio de Investigación Microbiológica, Universidad de Talca, Talca, Chile. Hubert, E.; Poblete, F. Instituto de Biología Vegetal y Biotecnología, Universidad de Talca, Talca, Chile. Navarro, A.; Nuñez, L. Departamento de Salud Pública, Universidad de Talca, Talca, Chile.
URI: http://dspace.utalca.cl/handle/1950/4069
ISSN: 0924-8579
Appears in Collections:Artículos en publicaciones ISI - Universidad de Talca

Files in This Item:

File Description SizeFormat
Full Text.htm2.87 kBHTMLView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Valid XHTML 1.0! DSpace Software Copyright © 2002-2009  The DSpace Foundation - Feedback