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Please use this identifier to cite or link to this item: http://dspace.utalca.cl/handle/1950/4168

Title: Free-radical Scavengers and Antioxidants from Peumus boldus Mol. (
Authors: Schmeda-Hirschmann, G.
Rodriguez, J.
Theoduloz, C.
Astudillo, L.
Feresin, G.E.
Tapia, A.
Keywords: Peumus boldus; Catechin; Boldine; Lipid peroxidation; Free-radical scavengers
Issue Date: 2003
Publisher: Taylor & Francis Ltd.
Citation: Free Radical Research 37(4): 447-452
Abstract: The dry leaves of Peumus boldus (Monimiaceae) are used in infusion or decoction as a digestive and to improve hepatic complains. Preliminary assays showed free-radical scavenging activity in hot water extracts of boldo leaves, measured by the decoloration of a methanolic solution of the 1,1-diphenyl-2-picryl-hydrazyl radical (DPPH). Assay-guided isolation led to the active compounds. Catechin proved to be the main free-radical scavenger of the extracts. Lipid peroxidation in erythrocytes was inhibited by boldo extracts and fractions at 500μg/ml with higher effect for the ethyl acetate soluble and alkaloid fractions. The IC[sub50] for catechin and boldine in the lipid peroxidation test were 75.6 and 12.5 μg/ml, respectively. On the basis of dry starting material, the catechin content in the crude drug was 2.25% while the total alkaloid calculated as boldine was 0.06%. The activity of boldine was six times higher than catechin in the lipid peroxidation assay. However, the mean catechin:total alkaloid content ratio was 37:1. The relative concentration of alkaloids and phenolics in boldo leaves and their activity suggest that free-radical scavenging effect is mainly due to catechin and flavonoids and that antioxidant effect is mainly related with the catechin content. The high catechin content of boldo leaves and its bioactivity suggest that quality control of Boldo folium has to combine the analysis of catechin as well as their characteristic aporphine alkaloids.
Description: G. Schmeda-Hirschmann; L. Astudillo. Laboratorio de Qui­mica de Productos Naturales, Instituto de Qui­mica de Recursos Naturales, Universidad de Talca, Casilla 747, Talca, Chile; J. A. Rodriguez;C. Theoduloz. Departamento de Ciencias Biomedicas, Facultad de Ciencias de la Salud, Universidad de Talca, Casilla 747, Talca, Chile.
URI: http://dspace.utalca.cl/handle/1950/4168
ISSN: 1071-5762
Appears in Collections:Artículos en publicaciones ISI - Universidad de Talca

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