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Please use this identifier to cite or link to this item: http://dspace.utalca.cl/handle/1950/4180

Title: Bioactive Metabolites from the Fungus Nectria galligena, the Main Apple Canker Agent in Chile
Authors: Gutierrez, M.
Theoduloz, C.
Rodriguez, J.
Lolas, M.
Schmeda-Hirschmann, G.
Keywords: Nectria galligena; Ascomycete; apple canker; colletochlorin B; colletorin B; ilicicolin C, E, and F; ,-dehydrocurvularin; biological activity
Issue Date: 2005
Publisher: American Chemical Society
Citation: Journal of agricultural and food chemistry 53 (20): 7701-7708
Abstract: The phytopathogenic fungus Nectria galligena Bres. is the most common canker disease agent of hardwood trees. The terpenoids colletochlorin B, colletorin B, ilicicolin C, E, and F, as well as the phytotoxin ,-dehydrocurvularin have been isolated from liquid cultures of N. galligena obtained from the xylem of infected apple trees in central Chile. Ilicicolin C and F and ,-dehydrocurvularin were active against Pseudomonas syringae with IC50 values of 28.5, 28.5, and 14.2 g/mL, respectively, in the same range as streptomycin and penicillin G (11 and 15 g/mL, respectively). All of the compounds showed moderate inhibitory activity toward the enzymes acetylcholinesterase (AChE) and -glucuronidase. The most active enzyme inhibitors were colletochlorin B and ilicicolin C and E, with IC50 values of 30-36 g/mL in the AChE assay and 32-43 g/mL in the -glucuronidase test. All of the chlorinated compounds showed some toxicity toward human lung fibroblasts, with IC50 values in the range of 64-120 g/mL. ,-Dehydrocurvularin proved to be the most toxic compound, showing IC50 values less than 12 g/mL. The effect of the isolated compounds on seed germination and radicle and epicotyl growth was assessed in lettuce and millet seeds. At 100 and 200 g/disk, ,-dehydrocurvularin significantly reduced radicle length and epicotyl growth in Lactuca sativa. This is the first report on the occurrence of colletochlorin B, colletorin B, ilicicolin C, E, and F, as well as ,-dehydrocurvularin associated to N. galligena.
Description: Laboratorio de Química de Productos Naturales, Instituto de Química de Recursos Naturales, Departamento de Ciencias Básicas Biomédicas, Facultad de Ciencias de la Salud, and Laboratorio de Fitopatología, Universidad de Talca, Talca, Chile.
URI: http://dspace.utalca.cl/handle/1950/4180
ISSN: 0021-8561
Appears in Collections:Artículos en publicaciones ISI - Universidad de Talca

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