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Please use this identifier to cite or link to this item: http://dspace.utalca.cl/handle/1950/4181

Title: Bioguided Isolation and Identification of the Nonvolatile Antioxidant Compounds from Fennel (Foeniculum vulgare Mill.) Waste
Authors: Parejo, I.
Viladomat, F.
Bastida, J.
Schmeda-Hirschmann, G.
Burillo, J.
Codina, C.
Keywords: Foeniculum vulgare; bitter fennel; Apiaceae; radical scavenging activity; bioguided isolation; phenolic compounds; LC/APCI-MS
Issue Date: 2004
Publisher: American Chemical Society
Citation: Journal of agricultural and food chemistry 52 (7): 1890 -1897
Abstract: A bioguided isolation of an aqueous extract of fennel waste led to the isolation of 12 major phenolic compounds. Liquid chromatography coupled to atmospheric pressure chemical ionization mass spectrometry (LC/UV/APCI-MS) combined with spectroscopic methods (NMR) was used for compound identification. Radical scavenging activity was tested using three methods: DPPH, superoxide nitro-blue tetrazolium hypoxanthine/xanthine oxidase, and OH/luminol chemiluminescence. In addition to products described in the literature, eight antioxidant compounds were isolated and identified for the first time in fennel: 3-caffeoylquinic acid, 4-caffeoylquinic acid, 1,5-O-dicaffeoylquinic acid, rosmarinic acid, eriodictyol-7-O-rutinoside, quercetin-3-O-galactoside, kaempferol-3-O-rutinoside, and kaempferol-3-O-glucoside. The structures of eriodictyol-7-O-rutinoside and quercetin-3-O-glucuronide were completely elucidated by two-dimensional NMR experiments. The isolated compounds exhibited a strong antiradical scavenging activity, which may contribute to the interpretation of the pharmacological effects of fennel.
Description: Schmeda-Hirschmann, G. Laboratorio de Química de Productos Naturales, Instituto de Química de Recursos Naturales.
URI: http://dspace.utalca.cl/handle/1950/4181
ISSN: 0021-8561
Appears in Collections:Artículos en publicaciones ISI - Universidad de Talca

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