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Please use this identifier to cite or link to this item: http://dspace.utalca.cl/handle/1950/4184

Title: Expression of a Bacillus thuringiensisδ-endotoxin cry1Ab gene in Bacillus subtilis and Bacillus licheniformis strains that naturally colonize the phylloplane of tomato plants (Lycopersicon esculentum, Mills)
Authors: Theoduloz, C.
Vega, A.
Salazar, M.
Gonzalez, E.
Meza-Basso, L.
Keywords: Bacillus thuringiensis; Bacillus subtilis; Bacillus licheniformis; Tuta absoluta; cry proteins; phylloplane; survival.
Issue Date: 2003
Publisher: Blackwell Publishing, Inc.
Citation: Journal of Applied Microbiology 94 (3), 375–381.
Abstract: Aims: To introduce a cry gene into microorganisms that naturally colonize the phylloplane of tomato plants to improve the persistence of the Cry proteins for controlling a South American tomato moth (Tuta absoluta, Meyrick, 1917). Methods and Results: A cry1Ab gene isolated from a native Bacillus thuringiensis strain (LM-466), showing a relevant activity against T. absoluta larvae, was cloned into the shuttle vector pHT315 (Arantes and Lereclus 1991). The construct was introduced by electroporation into native Bacillus subtilis and Bacillus licheniformis strains, both natural inhabitants of the tomato phylloplane. Western analysis and toxicity assays against the target larvae proved that the successful expression of the gene was accomplished in host bacteria. Recombinant toxin displayed a similar LC50 value in comparison to native donor strain LM-466. Both transformed Bacillus survived for at least 45 days on the tomato leaf surface. Conclusions: Plant-associated microorganisms that naturally colonize the phylloplane could be useful as recombinant microbial delivery systems of toxin genes of B. thuringiensis. Significance and Impact of the Study: Modified microorganisms capable of surviving on leaf surfaces for several weeks with insecticidal activity should allow for a reduction in pesticide application.
Description: C. Theoduloz, A. Vega, M. Salazar, E. González and L. Meza-Basso. Instituto de Biología Vegetal y Biotecnología, Universidad de Talca, Casilla, Talca, Chile. L. Meza-Basso, Instituto de Biología Vegetal y Biotecnología, Universidad de Talca, Casilla 747, Talca, Chile (e-mail: mezabas@pehuenche.utalca.cl).
URI: http://dspace.utalca.cl/handle/1950/4184
ISSN: 1364-5072
Appears in Collections:Artículos en publicaciones ISI - Universidad de Talca

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