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Please use this identifier to cite or link to this item: http://dspace.utalca.cl/handle/1950/4321

Title: Free Radical Scavengers and Antioxidants from Tagetes mendocina
Authors: Schmeda-Hirschmann, G.
Tapia, A.
Theoduloz, C.
Rodriguez, J.
Lopez, S.
Feresin, G.E.
Keywords: Tagetes mendocina; Phenolics; Antioxidant Activity
Issue Date: 2004
Publisher: Verlag Z Naturforsch
Citation: Zeitschrift Fur Naturforschung C-A Journal of Biosciences 591(5-6): 345-353
Abstract: Tagetes mendocina (Asteraceae) is a medicinal plant widely used in the Andean provinces of Argentina. Preliminary assays showed free radical scavenging activity in the methanol extract of the aerial parts, measured by the decoloration of a methanolic solution of the 1,1- diphenyl-2-picrylhydrazyl radical (DPPH) and scavenging of the superoxide anion. Assayguided isolation led to 4-hydroxyacetophenone (1), protocatechuic acid (2), syringic acid (3), patuletin (4), quercetagetin 7-O--d-glucoside (5), patuletin 7-O--d-glucoside (6) and axillarin 7-O--d-glucoside (7) as the free radical scavengers and antioxidant compounds from Tagetes mendocina. On the basis of dry startingmaterial, the total phenolic content of the crude drugwas 3.00% with 0.372% of flavonoids. The content of compounds 1Ð7 in the crude drugwas 0.008, 0.015, 0.010, 0.029, 0.238, 0.058 and 0.017%, respectively. Quercetagetin 7-O--d-glucoside proved to be the main free radical scavenger of the extracts measured by the DPPH decoloration test as well as for quenchingthe superoxide anion and inhibition of lipoperoxidation in erythrocytes. In the lipid peroxidation assay the percentual inhibition was related with the number of methoxy groups in the molecule, ranging from 86% for the quercetagetin glucoside to 67% for the monomethoxylated and 31% for the dimethoxylated derivative. The compounds showed low cytotoxicity towards human lungfibroblasts with IC50 > 1mm for compounds 1Ð3 and 0.24 to 0.52 mm for the flavonoids 4Ð7.
Description: Schmeda-Hirschmann, G. ; Tapia, A. Universidad de Talca, Instituto de Quımica de Recursos Naturales, Laboratorio de Productos Naturales, Casilla 747, Talca, Chile. Theoduloz, C. ;Rodriguez, J. Universidad de Talca, Departamento de Ciencias Basicas Biome dicas, Facultad de Ciencias de la Salud, Casilla 747, Talca, Chile.
URI: http://dspace.utalca.cl/handle/1950/4321
ISSN: 0939-5075
Appears in Collections:Artículos en publicaciones ISI - Universidad de Talca

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