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Please use this identifier to cite or link to this item: http://dspace.utalca.cl/handle/1950/4509

Title: Optimisation of Protoplast Production in White Lupin
Authors: Sinha, A.
Wetten, A.C.
Caligari, P.D.S.
Keywords: Lupinus albus; protoplast isolation; protoplast viability; protoplast yield
Issue Date: 2003
Publisher: Springer Netherlands
Citation: Biologia Plantarum 47(1): 21-25
Abstract: The influence, was investigated, of abiotic parameters on the isolation of protoplasts from in vitro seedling cotyledons of white lupin. The protoplasts were found to be competent in withstanding a wide range of osmotic potentials of the enzyme medium, however, −2.25 MPa (0.5 M mannitol), resulted in the highest yield of protoplasts. The pH of the isolation medium also had a profound effect on protoplast production. Vacuum infiltration of the enzyme solution into the cotyledon tissue resulted in a progressive drop in the yield of protoplasts. The speed and duration of orbital agitation of the cotyledon tissue played a significant role in the release of protoplasts and a two step (stationary-gyratory) regime was found to be better than the gyratory-only system.
Description: Caligari, P.D.S. Instituto Biología Vegetal y Biotecnología, Universidad de Talca, 2 Norte 685, Talca, Chile.
URI: http://dspace.utalca.cl/handle/1950/4509
ISSN: 0006-3134
Appears in Collections:Artículos en publicaciones ISI - Universidad de Talca

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