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Please use this identifier to cite or link to this item: http://dspace.utalca.cl/handle/1950/4550

Title: Highly heterogeneous families of Tyl/copia retrotransposons in the Lycopersicon chilense genome
Authors: Yanez, M.
Verdugo, I.
Rodriguez, M.
Prat, S.
Ruíz-Lara, S.
Keywords: transposable elements; repetitive DNA; gene expression; retroelements; tomato;
Issue Date: 1998
Publisher: Elsevier Science B.V.
Citation: Gene 222 (2): 223 - 228
Abstract: We have used the degenerated oligonucleotide primers-PCR (DOP-PCR) technique to determine the presence of Ty1/copia-related retrotransposons in the wild species of tomato, Lycopersicon chilense. Using degenerated oligonucleotides corresponding to highly conserved domains in the Ty1/copia retrotransposons, fragments of roughly 300 bp were obtained by PCR amplification. These were cloned in a plasmid vector and the nucleotide sequence determined for 20 clones, 19 of which showed sequence homology to retrotransposon-related sequences. Comparison of the deduced amino-acid sequence of these cloneswith those reported for other retrotransposons has allowed their classification into four distinct families: TLC1-TLC4. The level of aminoacid sequence similarity between these elements extends from 66.7% (between TLC1 and TLC2) to 42.6% (between TLC2 and TLC3). Altogether, the four families comprise about 0.17% of the L. chilense genome. RT-PCR analysis shows that the four TLC families are transcriptionally active, suggesting a mechanism for the generation of the observed diversity between the L, chilense retrotransposons.
Description: Mónica Yañez, Isabel Verdugo, Mariana Rodríguez, and Simón Ruiz-Lara. Instituto de Biología Vegetal y Biotecnología, Universidad de Talca, Casilla, 747 Talca, Chile.
URI: http://dspace.utalca.cl/handle/1950/4550
ISSN: 0378-1119
Appears in Collections:Artículos en publicaciones ISI - Universidad de Talca

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