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|Title: ||Highly heterogeneous families of Tyl/copia retrotransposons in the Lycopersicon chilense genome|
|Authors: ||Yanez, M.|
|Keywords: ||transposable elements; repetitive DNA; gene expression; retroelements; tomato;|
|Issue Date: ||1998 |
|Publisher: ||Elsevier Science B.V.|
|Citation: ||Gene 222 (2): 223 - 228|
|Abstract: ||We have used the degenerated oligonucleotide primers-PCR (DOP-PCR) technique to determine the presence of Ty1/copia-related retrotransposons in the wild species of tomato, Lycopersicon chilense. Using degenerated oligonucleotides corresponding to highly conserved domains in the Ty1/copia retrotransposons, fragments of roughly 300 bp were obtained by PCR amplification. These were cloned in a plasmid vector and the nucleotide sequence determined for 20 clones, 19 of which showed sequence homology to retrotransposon-related sequences. Comparison of the deduced amino-acid sequence of these cloneswith those reported for other retrotransposons has allowed their classification into four distinct families: TLC1-TLC4. The level of aminoacid sequence similarity between these elements extends from 66.7% (between TLC1 and TLC2) to 42.6% (between TLC2 and TLC3). Altogether, the four families comprise about 0.17% of the L. chilense genome. RT-PCR analysis shows that the four TLC families are transcriptionally active, suggesting a mechanism for the generation of the observed diversity between the L, chilense retrotransposons.|
|Description: ||Mónica Yañez, Isabel Verdugo, Mariana Rodríguez, and Simón Ruiz-Lara.
Instituto de Biología Vegetal y Biotecnología, Universidad de Talca, Casilla, 747 Talca, Chile.|
|Appears in Collections:||Artículos en publicaciones ISI - Universidad de Talca|
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