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Please use this identifier to cite or link to this item: http://dspace.utalca.cl/handle/1950/4572

Title: Differential expression of isoproterenol-induced salivary polypeptides in two mouse strains that are congenic for the H-2 histocompatibility gene complex
Authors: Solis, R.O.L.
Weis, U.K.
Ceballos, A.R.
Salas, G.H.
Keywords: saliva; salivary gland, polypeptides; strain, mouse, isoproterenol; secretion; proline-rich proteins
Issue Date: 2003
Publisher: John Wiley & Sons, Inc.
Citation: Journal of Cellular Biochemistry 90 (5): 945 - 956
Abstract: Two inbred mouse strains, A/Snell and A.Swiss, which were produced as congenic with regard to the H-2 histocompatibility gene complex, are homozygous for two different groups of isoproterenol-induced salivary polypeptides (IISP). These polypeptides, which have been considered as markers of the hypertrophic growth of the parotid acinar cells, are members of the complex family of salivary proline-rich proteins (PRP) on the basis of both their massive accumulation in the parotid acinar cells in response to chronic isoproterenol, secretory character, high solubility in trichloroacetic acid and metachromatic staining by Coomassie blue. IISP expressed in both mouse strains were identified by unidimensional SDS-polyacrylamide electrophoresis and Coomassie blue staining both in parotid gland homogenates and in whole salivas obtained from mice repeatedly stimulated at 24-h intervals with isoproterenol. Parotid glands from 40 mice (20 A/Snell and 20 A.Swiss) and salivas from 270 mice (200 A/Snell and 70 A.Swiss) were analyzed. One of the congenic strains (A/Snell) expressed five IISP (Mr 65, 61, 51.5, 38, and 37 kDa) and the other strain (A.Swiss) expressed six IISP (Mr 59, 57, 54.5, 46, 36, and 34 kDa). No inter-individual
Description: Ulrike Kemmerling Weis .Faculty of Health Sciences, University of Talca, Talca, Chile.
URI: http://dspace.utalca.cl/handle/1950/4572
ISSN: 0730-2312
Appears in Collections:Artículos en publicaciones ISI - Universidad de Talca

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