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| Title: | Detection and Classification of Trypanosoma cruzi by DNA Hybridization with Nonradioactive Probes |
| Authors: | Solari, A.
Venegas, J.
Gonzalez, E.
Vasquez, C. |
| Issue Date: | 1991 |
| Publisher: | Blackwell Publishing, Inc. |
| Citation: | The Journal of Eukaryotic Microbiology 38 (6): 559-565 |
| Abstract: | Total or kinetoplast DNA (kDNA) from 72 isolates and clones of Trypanosoma cruzi as well as from nine related trypanosomatids were analyzed by dot hybridization using nonradioactive kDNA or cloned minicircle fragments as probes. Biotinylated-kDNA probes generated by nick-translation proved reliable for distinguishing Zymodeme 1 and Zymodeme 2bol of T. cruzi parasites. In contrast, digoxigenin-labeled kDNA obtained by random-priming did not distinguish among T. cruzi isolates but did distinguish among New World leishmanias. Cloned minicircle fragments labeled with digoxigenin gave the same results as digoxigenin-labeled kDNA, except for a 10-fold decrease in sensitivity. Digoxigenin-labeled DNA probes proved useful in unambiguously detecting T. cruzi from different geographic regions of America. However, T. rangeli and T. cruzi marinkellei were not distinguished by these probes. |
| Description: | Gonzalez, E; Vasquez, C. Departamento de Ciencias Biologicas, Facultad de Recursos Naturales, Universidad de Talca, 2 Norte 685, Casilla 747, Talca, Chile. |
| URI: | http://dspace.utalca.cl/handle/1950/4599 |
| Appears in Collections: | Artículos en publicaciones ISI - Universidad de Talca
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