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Please use this identifier to cite or link to this item: http://dspace.utalca.cl/handle/1950/4609

Title: Coexistence of two chromatin structures in sperm nuclei of the bivalve molluscProtothaca thaca
Authors: Olivares, C.
Vera, M.L.
Ruíz Lara, Simón
Keywords: bivalve mollusc; sperm-specific protein; chromatin structure
Issue Date: 1993
Publisher: Springer Netherlands
Citation: Molecular and Cellular Biochemistry 125 (1): 87-95
Abstract: The chromatin of the spermatozoa from the bivalve molluscProtothaca thaca, has a peculiar composition in which coexist core histones with sperm-specific proteins H1 and Pt1, the latter being a protein exhibiting features intermediate between histones and protamines. In this paper, we report an analysis of chromatin organization using micrococcal nuclease digestion, salt fractionation of soluble chromatin derived from nuclease digestion and crosslinking experiments. The results obtained indicate that it is possible to obtain two types of chromatin, one which is soluble, more accessible to micrococcal nuclease action and which does not contain Pt1, and another insoluble type, more resistant to micrococcal nuclease and enriched in protein Pt1. The crosslinking experiments show that the protein Pt1 interacts with itself and with core histones but not with sperm-specific H1. These results have led us to propose a special structural arrangement for this chromatin. Based in the data reported here we propose the coexstence in the genome ofP. thaca of two interspersed chromatin domains, one nucleosomal and the other nonnucleosomal containing protein Pt1.
Description: Ruiz Lara, S. Departamento Ciencias Biológicas, Universidad de Talca, Talca, Chile.
URI: http://dspace.utalca.cl/handle/1950/4609
ISSN: 0300-8177
Appears in Collections:Artículos en publicaciones ISI - Universidad de Talca

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