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Please use this identifier to cite or link to this item: http://dspace.utalca.cl/handle/1950/4628

Title: Two related, low-temperature-induced genes from Brassica napus are homologous to the human tumour bbc1 (breast basic conserved) gene
Authors: Saez Vasquez, J.
Raynal, M.
Meza-Basso, L.
Delseny, M.
Keywords: Brassica napus; cold acclimation; gene isolation;human tumour
Issue Date: 1993
Publisher: Springer Netherlands
Citation: Plant Molecular Biology 23 (6): 1211-1221
Abstract: In order to identify genes involved in cold acclimation, we have constructed a cDNA library from Brassica napus (cv. Samouraï) cold-acclimated etiolated seedlings. By differential screening, a cDNA clone named pBnC24 (Brassica napus Cold), corresponding to a new cold-inducible plant gene, was isolated. Northern blot hybridizations using total RNA from acclimated and unacclimated seedlings confirmed that BnC24 represents a cold-regulated gene. In contrast with a number of cold-inducible plant genes, BnC24 does not seem to be responsive to abscisic acid (ABA). In addition, further screening of the cold-acclimated cDNA library using pBnC24 cDNA as a probe, allowed the isolation of a second type of homologous cDNA. Sequence analysis showed that the two BnC24 genes encode basic 24 kDa proteins, which are highly hydrophilic and rich in alanine, lysine and arginine. The nucleotide and deduced amino acid sequences of these clones do not show any homology with other previously described cold-induced plants genes. However they have strong homology with a recently discovered human tumour gene, bbc1 (breast basic conserved), which seems to be highly conserved in eukaryotes.
Description: Luis Meza-Basso. Departamento de Ciencias Biologicas, Programa de Biología vegetal, Universidad de Talca, 2 Norte 685, Casilla 747, Talca, Chile.
URI: http://dspace.utalca.cl/handle/1950/4628
ISSN: 0167-4412
Appears in Collections:Artículos en publicaciones ISI - Universidad de Talca

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