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Please use this identifier to cite or link to this item: http://dspace.utalca.cl/handle/1950/4800

Title: Free radical scavenging activity and secondary metabolites from in vitro cultures of Sanicula graveolens
Authors: Cheel, J.
Schmeda-Hirschmann, G.
Jordan, M.
Theoduloz, C.
Rodriguez, J.
Gerth, A.
Wilken, D.
Keywords: In vitro Propagation, Secondary Metabolite Content, Sanicula graveolens
Issue Date: 2007
Publisher: Verlag Z Naturforsch
Citation: Zeitschrift für Naturforschung. C, A Journal of Biosciences 62 (7-8): 555 -562
Abstract: An in vitro propagation system was developed to obtain shoot and root cultures from the Andean spice Sanicula graveolens (Apiaceae). Propagation of shoots, roots and plantlets was achieved by the temporary immersion system. The free radical scavenging effect of the methanol/ water (7 :3 v/v) extracts was determined by the discoloration of the 1,1-diphenyl-2-picrylhydrazyl radical (DPPH). Total phenolic, flavonoid, chlorogenic acid (CA) and quercetin 3-O-glucoside content in the samples was assessed by spectrophotometry and DAD-HPLC analysis, respectively. On a dry weight basis, the crude extracts showed total phenolic values ranging from 3.57 to 6.93%, with highest content for the root culture sample. Total flavonoid content ranged from 1.23 to 2.23% and was lower for the root culture. Chlorogenic acid and neochlorogenic acid were identified by TLC in all samples. Highest free radical scavenging effect was observed for the root culture which also presented the highest CA content. Two of the shoot culture samples, with similar IC50 values in the DPPH discoloration assay, also presented close quercetin-3-O-glucoside content.
Description: Jose Cheel; Guillermo Schmeda-Hirschmanna; Laboratorio de Quımica de Productos Naturales, Instituto de Quımica de Recursos Naturales, Universidad de Talca, Casilla 747, Talca, Chile. Fax: +5671-200448. Jaime A. Rodrıguez,Facultad de Ciencias de la Salud, Universidad de Talca, Casilla 747, Talca, Chile.
URI: http://dspace.utalca.cl/handle/1950/4800
ISSN: 0939-5075
Appears in Collections:Artículos en publicaciones ISI - Universidad de Talca

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