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Please use this identifier to cite or link to this item: http://dspace.utalca.cl/handle/1950/4813

Title: Calcium release by ryanodine receptors mediates hydrogen peroxide-induced activation of ERK and CREB phosphorylation in N2a cells and hippocampal neurons
Authors: Kemmerling, U.
Munoz, P
Muller, M.
Sanchez, G.
Aylwin, M.L.
Klann, E.
Carrasco, M.A.
Hidalgo, C.
Keywords: Hippocampus; Reactive oxygen species; Redox modifications; S-glutathionylation; Intracellular stores; Calcium release channels; Synaptic plasticity
Issue Date: 2007
Publisher: Elsevier Ltd
Citation: Cell Calcium 41 (5): 491-502
Abstract: Hydrogen peroxide, which stimulates ERK phosphorylation and synaptic plasticity in hippocampal neurons, has also been shown to stimulate calcium release in muscle cells by promoting ryanodine receptor redox modification (S-glutathionylation). We report here that exposure of N2a cells or rat hippocampal neurons in culture to 200 μM H2O2 elicited calcium signals, increased ryanodine receptor S-glutathionylation, and enhanced both ERK and CREB phosphorylation. In mouse hippocampal slices, H2O2 (1 μM) also stimulated ERK and CREB phosphorylation. Preincubation with ryanodine (50 μM) largely prevented the effects of H2O2 on calcium signals and ERK/CREB phosphorylation. In N2a cells, the ERK kinase inhibitor U0126 suppressed ERK phosphorylation and abolished the stimulation of CREB phosphorylation produced by H2O2, suggesting that H2O2 enhanced CREB phosphorylation via ERK activation. In N2a cells in calcium-free media, 200 μM H2O2 stimulated ERK and CREB phosphorylation, while preincubation with thapsigargin prevented these enhancements. These combined results strongly suggest that H2O2 promotes ryanodine receptors redox modification; the resulting calcium release signals, by enhancing ERK activity, would increase CREB phosphorylation. We propose that ryanodine receptor stimulation by activity-generated redox species produces calcium release signals that may contribute significantly to hippocampal synaptic plasticity, including plasticity that requires long-lasting ERK-dependent CREB phosphorylation.
Description: Ulrike Kemmerling. Facultad de Ciencias de la Salud, Universidad de Talca, Talca, Chile.
URI: http://dspace.utalca.cl/handle/1950/4813
ISSN: 0143-4160
Appears in Collections:Artículos en publicaciones ISI - Universidad de Talca

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