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Please use this identifier to cite or link to this item: http://dspace.utalca.cl/handle/1950/4904

Title: Cloning the BST(VI) retriction- modification system in Escherichia coli
Authors: Vasquez, C.
Saavedra, C.
Gonzalez, E.
Keywords: Restriction Endonuclease; modification methyltransferase; bacillus-stearothermophilus; heterologous gene expression; recombinant dna
Issue Date: 1991
Publisher: Elsevier Science B.V.
Citation: Gene 102 (1): 83-85
Abstract: A standard DNA modification methyltransferase (MTase) selection protocol was followed to clone the BstVI restriction and modification system from Bacillus stearothermophilus in Escherichia coli. Both genes were contained in a 4.4-kb EcoRI fragment from B. stearothermophilus V chromosomal DNA. The heterologous expression of these genes did not depend on their orientation in the vector, suggesting that the genes are expressed in E. coli under the control of promoters located on the cloned fragment. Subcloning experiments demonstrated that the bstVIR gene was expressed in the absence of its cognate MTase.
Description: Enrique González and Claudio Vásquez Laboratorio de Biología Molecular, Departamento de Ciencias Biológicas, Universidad de Talca, Casilla 747, Talca, Chile.
URI: http://dspace.utalca.cl/handle/1950/4904
ISSN: 0378-1119
Appears in Collections:Artículos en publicaciones ISI - Universidad de Talca

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