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Please use this identifier to cite or link to this item: http://dspace.utalca.cl/handle/1950/8811

Title: In vitro propagation of cedar (Cedrela odorata L.) from juvenile shoots
Authors: Garcia-Gonzales, R.
Delgado, M.
Gonzalez, Y.
Gonzalez, A.
Garriga, M.
Caligari, P.D.S.
Carrasco, B.
Quiroz, K.
Keywords: Organogenesis
in vitro propagation
Cedrela odorata
in vitro rooting
plant growth regulators
Issue Date: 2011
Citation: CHILEAN JOURNAL OF AGRICULTURAL RESEARCH Volume: 71 Issue: 3 Pages: 376-382
Abstract: Cedrela odorata L. is one of the most important timber species currently traded in the Caribbean and Central America; however, it has been intensively exploited. In vitro techniques and clonal propagation can help to develop new plantations and assist in establishing improvement programs for this species. The aim of this study was to develop a protocol to establish in vitro conditions and to micropropagate this species from nodal explants from juvenile cuttings taken from field trees. Disinfection of node explants with 5% propiconazole CE 25 during 3 min resulted in 100% explant disinfection and 60% morphogenic response on those established explants. Shoot development was optimized by cultivating in vitro node explants in Murashige and Skoog basal medium supplemented with 2 mg L(-1) 6-bencilaminopurine and 3 mg L(-1) naphthaleneacetic acid. This medium resulted in 100% shoot development from the in vitro node explants with a 3.93 cm mean height. Rooting was also stimulated 6 wk after individualization of the regenerated plants on the same micropropagation medium with a mean of 3.9 roots per plant. In vitro plants did not show morphologic differences when compared to ex vitro seeds.
Description: Garriga, M (Garriga, Miguel); Caligari, PDS (Caligari, Peter D. S.). Univ Talca, Inst Biol Vegetal & Biotecnol, Talca, Chile
URI: http://dspace.utalca.cl/handle/1950/8811
Appears in Collections:Artículos en publicaciones ISI - Universidad de Talca

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