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Please use this identifier to cite or link to this item: http://dspace.utalca.cl/handle/1950/8826

Title: rypanosoma cruzi induces apoptosis in ex vivo infected human chorionic villi
Authors: Duaso, J.
Rojo, G.
Jana, E.
Galanti, N.
Cabrera, G.
Bosco, C.
Lopez-Munoz, R.
Maya, J.D.
Ferreira, J.
Kemmerling, U.
Keywords: Trypanosoma cruzi
Apoptosis
Human chorionic villi
Issue Date: May-2011
Publisher: W B SAUNDERS CO LTD
Citation: LACENTA Volume: 32 Issue: 5 Pages: 356-361 DOI: 10.1016/j.placenta.2011.02.005
Abstract: Chagas' disease, produced by the haemoflagellated protozoan Trypanosoma cruzi (T. cruzi), is one of the most frequent endemic diseases in Latin America. In spite that in the past few years T. cruzi congenital transmission has become of epidemiological importance, studies about this mechanism of infection are scarce. The placental tissue undergoes apoptosis throughout gestation, as part of its normal turnover. On the other hand, it is known that T cruzi induces, delays or inhibits apoptosis in other mammalian tissues. In order to determine the effect of parasite invasion on normal apoptosis in the placenta, explants of human chorionic villi were incubated with 105 trypomastigotes for 24 h. Effective infection was tested by visualizing T cruzi antigens in histological preparations and by PCR. Upon infection, apoptotic cell death was determined by light and transmission electron microscopy, TUNEL analysis, measurement of caspase-3 like activity and immunohistochemical detection of caspase 3 cleaved cytokeratin 18. Our results clearly show that T cruzi induces apoptosis in the chorionic villi and suggest that this is one of mechanisms used by the parasite to insure infection and invasion of human placenta and fetus. (C) 2011 Elsevier Ltd. All rights reserved
Description: Kemmerling, U (Kemmerling, U.). Univ Talca, Fac Ciencias Salud, Dept Estomatol, Talca, Chile
URI: http://dspace.utalca.cl/handle/1950/8826
ISSN: 0143-4004
Appears in Collections:Artículos en publicaciones no ISI - Universidad de Talca

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